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Updated: Sep 27, 2025
Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells
Published on: August 17, 2022
B lymphocytes subpopulations are associated with cardiac remodeling in elderly patients with advanced chronic kidney
Jieshan Lin1, Bin Tang1, Ganyuan He2
1Department of Nephrology, Blood Purification Center, Zhongshan City People's Hospital, Zhongshan 528403, China.
Insights
In advanced Chronic Kidney Disease (CKD), specific B lymphocyte populations (CD19(+)CD5(+) and CD19(+)CD5(-)) correlate with cardiac remodeling. Lower levels of these B cells are linked to increased mortality risk in CKD patients.
Area of Science:
- Nephrology
- Cardiology
- Immunology
Background:
- Chronic Kidney Disease (CKD) significantly elevates cardiovascular disease (CVD) risk.
- Cardiac remodeling is a key factor in CVD development, particularly in advanced CKD stages.
- The role of specific B lymphocyte subsets in CKD-associated cardiac remodeling requires further investigation.
Purpose of the Study:
- To investigate the association between B lymphocyte subsets (CD19(+)CD5(+) and CD19(+)CD5(-)) and cardiac remodeling in elderly patients with advanced CKD.
- To explore the relationship between these B cell populations and clinical markers of kidney disease and cardiac function.
Main Methods:
- A retrospective study involving 167 participants (84 advanced CKD patients, 83 controls).
- Identification of CD19(+)CD5(+) and CD19(+)CD5(-) B cell subsets using flow cytometry.
- Correlation analysis of B cell subsets with echocardiographic parameters and clinical data.
Main Results:
- CD19(+)CD5(+) and CD19(+)CD5(-) B cells showed negative correlations with markers of cardiac hypertrophy (e.g., LVM, LVSD) and positive correlations with left ventricular ejection fraction (LVEF).
- Lower counts of both B cell subsets were associated with higher levels of cardiac biomarkers (pro-BNP, TNT-hs) and increased risk of all-cause mortality.
- Hypertension was more prevalent in CKD patients compared to controls.
Conclusions:
- B lymphocyte subsets CD19(+)CD5(+) and CD19(+)CD5(-) are negatively associated with ventricular hypertrophy parameters in advanced CKD.
- These findings suggest a potential protective role for B lymphocytes in mitigating cardiac remodeling in CKD patients.
- B cell populations may be involved in the pathogenesis of cardiac complications in CKD.
Aim:
Chronic Kidney Disease (CKD) is independently associated with increased cardiovascular disease (CVD) risk. The aim of this study was to investigate the potential roles of B lymphocyte populations with cardiac remodeling in elderly patients with advanced CKD.
Methods:
We designed a retrospective study in a cohort of 167 patients (84 advanced CKD patients with stage 4-5 and 83 non-CKD controls). B cell subsets: CD19(+)CD5(+) and CD19(+)CD5(-) B cells were identified by flow cytometry. Correlation of B cells subsets with cardiac remodeling and clinical data in elderly CKD patients were analyzed.
Results:
In this study, we found that the prevalence of hypertension was more common in CKD patients than in the control subjects (P < 0.05). Spearman's analysis showed that CD19(+)CD5(+) B cells were negatively correlated with high sensitivity C-reactive protein (hsCRP), β2-microglobulin (β2-MG), serum creatinine (SCr), pro-brain natriuretic peptide (pro-BNP), high-sensitivity troponin T (TNT-hs), left ventricle end-diastolic dimension (LVDD), left ventricle end-systolic dimension (LVSD) and left ventricular mass (LVM), and CD19(+)CD5(-) B cells were negatively correlated with β2-MG, SCr, pro-BNP and TNT-hs (P < 0.05). In contrary, left ventricular ejection fractions (LVEF) was positively correlated with CD19(+)CD5(+) and CD19(+)CD5(-) B cells (P < 0.05). In addition, patients with higher levels of CD19(+)CD5(+) B cells exhibited lower level of pro-BNP, TNT-hs, interventricular septum (IVS), LVSD and LVM (P < 0.05). Higher levels of CD19(+)CD5(-) B cells also presented lower levels of pro-BNP, TNT-hs and LVSD, but higher levels of LVEF (P < 0.05). Cox regression analysis showed that patients with higher levels of LVSD, lower CD19(+)CD5(+)and CD19(+)CD5(-) B cells counts have a higher risk of all-cause mortality (P < 0.05).
Conclusions:
Our results showed that CD19(+)CD5(+) and CD19(+)CD5(-) B lymphocytes were negatively correlated with ventricular hypertrophy-related echocardiographic parameters in advanced CKD patients, which indicated that B lymphocytes might be involved in pathogenesis and improve cardiac remodeling in CKD patients.
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