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Neurite Outgrowth-Promoting Compounds from Cockscomb Hydrolysate.
1Graduate School of Advanced Technology and Science, Tokushima University, 2-1 Minamijosanjima-cho, Tokushima 770-8506, Japan.
Nutrients
|April 12, 2022
Summary
Cockscomb hydrolysate promotes neurite outgrowth in PC12 cells. This activity stems from a synergistic effect of the amino acids threonine, alanine, valine, and methionine.
Area of Science:
- Neuroscience
- Biochemistry
- Pharmacology
Background:
- Cockscomb hydrolysate exhibits neurite outgrowth-promoting activity in PC12 cells.
- Identifying the specific compounds responsible for this activity is crucial for understanding its mechanism.
Purpose of the Study:
- To isolate and identify the compounds in cockscomb hydrolysate responsible for promoting neurite outgrowth.
- To elucidate the mechanism underlying the neurite outgrowth-promoting activity.
Main Methods:
- Bioassay-guided purification involving liquid-liquid partition and column chromatography.
- High-performance liquid chromatography (HPLC) and proton nuclear magnetic resonance (1H NMR) for compound identification.
- Screening of individual amino acids and their mixtures for neurite outgrowth activity.
Main Results:
- Threonine, alanine, valine, and methionine were identified as the main compounds in the active fractions.
- Valine and methionine demonstrated individual neurite outgrowth-promoting activity.
- Alanine enhanced valine's activity, and a mixture of valine and methionine showed increased activity compared to individual components.
- Dipeptides of valine and methionine exhibited weak activity.
Conclusions:
- The potent neurite outgrowth-promoting activity of cockscomb hydrolysate is attributed to the synergistic interaction of threonine, alanine, valine, and methionine.
- Individual amino acids at concentrations found in the hydrolysate did not show significant activity, highlighting the importance of their combined effect.
