Detection of Pyrethroids in Food by Immunofluorescence Enhanced Method Based on Three-Layer Core-Shell Structure...

Area of Science:

• Materials Science
• Analytical Chemistry
• Nanotechnology

Background:

• Rare earth upconversion nanomaterials offer unique optical properties.
• Developing sensitive detection methods for pesticide residues is crucial for food safety.

Purpose of the Study:

• To synthesize a novel rare earth upconversion nanomaterial with enhanced efficiency.
• To establish an upconversion magnetic separation immunoassay for pyrethroid pesticide residue detection.

Main Methods:

• Synthesis of a three-layer sandwich core-shell upconversion nanomaterial via thermal decomposition.
• Characterization using TEM, XRD, and fluorescence spectrophotometry.
• Development of an immunoassay utilizing magnetic separation and fluorescence detection.

Main Results:

• The novel nanomaterial exhibited significantly improved upconversion luminous efficiency.
• The immunoassay achieved low limits of detection for fenpropathrin (0.01 μg/L), cypermethrin (0.015 μg/L), and fenvalerate (0.011 μg/L).
• High recovery rates (83.4–97.8%) were observed in real food samples (apple, cabbage).

Conclusions:

• The developed upconversion magnetic separation immunoassay is accurate and sensitive for pyrethroid residue analysis.
• This method provides a reliable tool for quantitative analysis of pyrethroids in food products.
• The novel nanomaterial shows promise for advanced analytical applications.