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Related Concept Videos

Centrifugation01:05

Centrifugation

2.6K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Related Experiment Video

Updated: Sep 27, 2025

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols.

Zahra Kargarpour1, Layla Panahipour1, Richard J Miron2,3

  • 1Department of Oral Biology, Medical University of Vienna, 1010 Vienna, Austria.

Molecules (Basel, Switzerland)
|April 12, 2022
PubMed
Summary

Liquid platelet-rich fibrin (PRF) contains fibrinogen, crucial for sticky bone preparation. Platelet-poor plasma (PPP) fraction is the primary source of clottable fibrinogen in PRF, not the buffy coat (BC).

Keywords:
blood clotblood coagulationbuffy coatfibrinogenplatelet-poor plasmaplatelet-rich fibrinsticky bone

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Area of Science:

  • Biomaterials Science
  • Hematology
  • Regenerative Medicine

Background:

  • Liquid platelet-rich fibrin (PRF) is a blood derivative used in regenerative medicine.
  • Its utility in preparing sticky bone relies on fibrinogen content, which remains unevaluated.
  • Understanding fibrinogen distribution in PRF fractions is essential for optimizing its clinical application.

Purpose of the Study:

  • To quantify fibrinogen concentration in different liquid PRF fractions.
  • To determine the proportion of clottable matrix in PRF fractions.
  • To elucidate the role of PRF fractions in sticky bone preparation.

Main Methods:

  • Liquid PRF was prepared at varying relative centrifugal force (RCF) levels (300, 700, 2000) for 8 minutes.
  • Fibrinogen levels were quantified using immunoassay.
  • The weight of clotted PRF fractions was measured before and after serum removal.

Main Results:

  • Fibrinogen concentration was consistently higher in the platelet-poor plasma (PPP) fraction compared to the buffy coat (BC) fraction.
  • Fibrinogen levels decreased further in the red blood cell fraction.
  • The clottable matrix constituted 10.2% of PPP and 25.3% of BC, with over half of BC's weight attributed to cellular components.

Conclusions:

  • Platelet-poor plasma (PPP) is the primary source of clottable fibrinogen in liquid PRF.
  • The buffy coat (BC) fraction serves more as a cellular component source than a fibrinogen source for sticky bone preparation.
  • These findings offer critical insights into optimizing PRF utilization in bone regeneration procedures.