LPS Response Is Impaired by Urban Fine Particulate Matter

Fine particulate matter (PM_2.5) is a complex mixture of components with diverse chemical and physical characteristics associated with increased respiratory and cardiovascular diseases mortality. Our study aimed to investigate the effects of exposure to concentrated PM_2.5 on LPS-induced lung injury onset. BALB/c male mice were exposed to either filtered air or ambient fine PM_2.5 in an ambient particle concentrator for 5 weeks. Then, an acute lung injury was induced with nebulized LPS. The animals were euthanized 24 h after the nebulization to either LPS or saline. Inflammatory cells and cytokines (IL-1β, IL-4, IL-5, IL-6, IL-10, IL-17, TNF) were assessed in the blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, lung morphology was assessed by stereological methods. Our results showed that the PM+LPS group showed histological evidence of injury, leukocytosis with increased neutrophils and macrophages, and a mixed inflammatory response profile, with increased KC, IL-6, IL-1β, IL-4, and IL-17. Our analysis shows that there is an interaction between the LPS nebulization and PM_2.5 exposure, differently modulating the inflammatory response, with a distinct response pattern as compared to LPS or PM_2.5 exposure alone. Further studies are required to explain the mechanism of immune modulation caused by PM_2.5 exposure.