MIG-seq is an effective method for high-throughput genotyping in wheat (Triticum spp.)

Area of Science:

• Agricultural Science
• Genomics
• Plant Breeding

Background:

• Multiplexed inter-simple sequence repeats genotyping by sequencing (MIG-seq) is a low-cost genotyping technology.
• Its application in agricultural plants is limited by the perceived low number of polymorphisms.
• The relationship between genome size and MIG-seq efficacy requires further investigation.

Purpose of the Study:

• To investigate the relationship between genome size and the number of stably sequenced bases using MIG-seq across 12 plant species.
• To evaluate the applicability of MIG-seq for population structure, linkage mapping, and quantitative trait loci (QTL) analysis in wheat.
• To demonstrate the effectiveness of MIG-seq for genomic analysis in large-genome agricultural plants.

Main Methods:

• Applied MIG-seq to 12 diverse plant species.
• Analyzed the correlation between genome size and the number of MIG-seq loci.
• Conducted population structure analysis, linkage mapping, and QTL analysis on wheat using MIG-seq data.

Main Results:

• A positive correlation was found between genome size and the number of loci sequenced by MIG-seq, attributed to the linkage between genome size and simple sequence repeats (SSRs).
• MIG-seq successfully differentiated populations of tetraploid wheat based on collection sites and subspecies.
• Over 3,000 SNPs/indels were detected in wheat mapping populations, and QTL analysis identified known flowering-related genes.

Conclusions:

• MIG-seq is effective for analyzing agricultural plants, even those with large genomes.
• The number of detectable polymorphisms via MIG-seq is positively linked to genome size.
• MIG-seq provides a valuable tool for genomic studies in diverse crop species, including wheat.