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Related Experiment Video

Updated: Sep 27, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Single molecule detection; from microscopy to sensors.

Nidhi Chauhan1, Kirti Saxena1, Utkarsh Jain1

  • 1Amity Institute of Nanotechnology (AINT), Amity University Uttar Pradesh (AUUP), Noida 201313, U.P., India.

International Journal of Biological Macromolecules
|April 12, 2022
PubMed
Summary
This summary is machine-generated.

Single molecule detection is crucial for understanding cellular mechanisms and advancing healthcare. This review highlights advanced biosensing technologies and traditional methods for highly sensitive single-molecule analysis.

Keywords:
BiosensorDetectionMicroscopyNanoporePlasmonSingle molecules

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Medical Diagnostics

Background:

  • Understanding cellular mechanisms requires precise analysis at the single-molecule level.
  • Single molecule detection offers new avenues for healthcare innovation.
  • Traditional and advanced techniques are essential for sensitive molecular analysis.

Purpose of the Study:

  • To review traditional and biosensing techniques for single molecule detection.
  • To highlight the sensitivity and applications of various detection methods.
  • To provide an overview of advancements in single molecule detection technologies.

Main Methods:

  • Plasmonic resonance
  • Nanopore sensing
  • Whispering gallery mode resonators
  • Simoa assay
  • Recognition tunneling
  • Surface-enhanced Raman scattering (SERS) biosensors
  • CRISPR/Cas biosensors

Main Results:

  • Various traditional methods offer single molecule sensitivity.
  • Biosensing technologies, including plasmonic, SERS, and CRISPR/Cas biosensors, are highly sensitive for single molecule detection.
  • Significant progress has been made in developing techniques for single molecule detection.

Conclusions:

  • Single molecule detection is vital for elucidating cellular functions and improving healthcare.
  • Biosensors represent a powerful approach for achieving high sensitivity in single molecule detection.
  • Continued advancements in detection techniques promise further breakthroughs in biological and medical research.