Cascade amplification strategy combined with analyte-triggered fluorescence switching of dual-quenching system for...

A sensitive fluorescence sensing platform consisting of manganese dioxide nanosheets (MnO₂) and gold nanoparticles (AuNPs) as dual nanoquenchers has been constructed to detect isoniazid combined with analyte-triggered cascade reactions. The fluorescence of 2,3-diaminophenazine (DAP) is quenched simultaneously by MnO₂ and AuNPs via inner filter effect. MnO₂ is decomposed by isoniazid to generate Mn²⁺, which makes AuNPs aggregated. The quenching abilities of both the decomposed MnO₂ and aggregated AuNPs are inhibited, causing remarkable fluorescence recovery. The usage of dual nanoquenchers enhances the quenching efficiency and reduces the fluorescence background. Moreover, the isoniazid-triggered cascade reaction further amplifies the readout signal. Thus, this strategy exhibits higher sensitivity towards the detection of isoniazid. Compared with MnO₂-based fluorescence assay, this strategy possesses lower limit of detection. This strategy has been successfully used to detect isoniazid in pharmaceutical preparations, which is of great significance for drug analysis.