Related Experiment Video

Updated: Sep 27, 2025

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients
09:31

Generation of Induced-pluripotent Stem Cells Using Fibroblast-like Synoviocytes Isolated from Joints of Rheumatoid Arthritis Patients

Published on: October 16, 2016

9.5K

Dynamic synovial fibroblasts are modulated by NBCn1 as a potential target in rheumatoid arthritis

Minjeong Ji1,2, Hee Jung Ryu3, Hyeon-Man Baek4,5

  • 1Department of Physiology, College of Medicine, Gachon University, Lee Gil Ya Cancer and Diabetes Institute, 155 Getbeolro, Yeonsu-gu, Incheon, South Korea.

Experimental & Molecular Medicine
|April 13, 2022
PubMed

Insights

Modulating sodium bicarbonate cotransporter n1 (NBCn1) in fibroblast-like synoviocytes (FLSs) reduced rheumatoid arthritis (RA) severity. Targeting NBCn1 offers a potential new therapeutic strategy for RA patients.

Area of Science:

  • Immunology
  • Cell Biology
  • Rheumatology

Background:

  • Rheumatoid arthritis (RA) is an autoimmune disease driven by aggressive fibroblast-like synoviocytes (FLSs) and pannus formation.
  • Current RA therapies target inflammatory cytokines, but FLS migration is a key factor in disease progression.
  • Investigating FLS migratory mechanisms offers potential for novel therapeutic strategies.

Purpose of the Study:

  • To investigate the role of FLS migration in RA pathogenesis.
  • To identify key molecular targets within the FLS migratory apparatus.
  • To evaluate the therapeutic potential of modulating FLS migration in an RA model.

Main Methods:

  • Primary RA-FLSs were cultured and stimulated with inflamed synovial fluid or TNF-α.
  • Sodium bicarbonate cotransporter n1 (NBCn1) expression and function were assessed.
  • FLS migration was inhibited using the NBC inhibitor S0859.
  • A collagen-induced arthritis (CIA) mouse model was used to evaluate therapeutic efficacy and toxicity.

Main Results:

  • Inflamed synovial fluid-stimulated RA-FLSs showed enhanced migration and increased expression of migratory apparatus components.
  • NBCn1 was identified in primary cultured RA-FLSs for the first time.
  • S0859 significantly attenuated RA-FLS migration induced by synovial fluid or TNF-α.
  • Inhibition of NBCs with S0859 in the CIA mouse model reduced joint swelling and destruction without significant toxicity.

Conclusions:

  • Inflammatory mediators recruit NBCn1 to the FLS plasma membrane, contributing to their migratory properties.
  • Modulation of NBCn1 represents a promising therapeutic strategy for rheumatoid arthritis.
  • Targeting NBCn1 could offer a novel approach to managing RA by inhibiting FLS migration and subsequent joint damage.

More Related Videos

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue09:18

Author Spotlight: Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Published on: February 24, 2023

8.6K
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.5K

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.4K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.2K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.9K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.8K
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
2.6K