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Fermentation01:29

Fermentation

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Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
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Grape stalk valorization for fermentation purposes.

Tuğba Atatoprak1, Maria Manuela Amorim1, Tânia Ribeiro1

  • 1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal.

Food Chemistry. Molecular Sciences
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Summary

Grape stalks can be converted into valuable sugars and ethanol using enzymatic hydrolysis and fermentation. This research demonstrates an integrated approach to maximize grape stalk biomass economic value.

Keywords:
BiosugarsCellulose nanocrystalsFermentationGrape stalkLignin

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Area of Science:

  • Biomass valorization
  • Sustainable chemistry
  • Fermentation technology

Background:

  • Grape stalks are an abundant agricultural byproduct.
  • Maximizing the economic value of grape waste is crucial for sustainability.

Purpose of the Study:

  • To develop an integrated process for grape stalk valorization.
  • To produce fermentable sugars and ethanol from grape stalks.
  • To create cellulose nanocrystals from residual biomass.

Main Methods:

  • Fractionation of white and red grape stalks.
  • Acid and enzymatic hydrolysis for sugar production.
  • Fermentation using *Pichia stipitis* and *Saccharomyces cerevisiae*.
  • Production of cellulose nanocrystals.

Main Results:

  • Enzymatic hydrolysis yielded higher sugar concentrations than dilute acid hydrolysis.
  • *Pichia stipitis* produced higher ethanol yields due to higher xylose content.
  • Cellulose nanocrystals were successfully produced from the residual biomass.

Conclusions:

  • Integrated valorization of grape stalks is feasible.
  • Enzymatic hydrolysis and specific yeast strains enhance sugar and ethanol production.
  • This approach offers a novel pathway for grape waste utilization.