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Cloning vectors for gene delivery, integration and expression in Campylobacter jejuni

Gaylen A Uhlich1, Lori Bagi1, Nereus W Gunther1

  • 1Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.

Biotechniques
|April 13, 2022
PubMed

Keywords:
Campylobacterfimbriaegene expressionplasmidsrestored function

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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