A hybrid boronate affinity probe for the selective detection of cis-diol containing compounds in tea beverages
Yunchun Liu1, Liyun Hua1, Wanru Zhu1
1Key Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo-Biosensing, Anhui Provincial Engineering Laboratory for New-Energy Vehicle Battery Energy-Storage Materials, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, P. R. China.
Abstract:
UiO-66-NH2 nanocomposite was post-modified with 4-mercaptophenylboronic acid (MPBA) by the method of in situ hybridization reaction. The hybrid boronate affinity material UiO-NH2 @P (TEPIC-co-MPBA) was characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. The material was applied as fluorescent probe for the detection of cis-diol containing compounds based on the boronate affinity mechanism, and exhibited high specific selectively. The proposed method exhibited good linearity for the detection of catechol in the range of 0.50 to 8.00 μg ml-1 . The detection limit was 0.13 μg ml-1 . The tactic was successfully applied to analyze the total polyphenols in tea beverages for catechol, and relative recovery was in 98.86-106.00%. Therefore, this work provided a promising strategy for the recognition of cis-diol containing compounds.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Diels–Alder Reaction: Characteristics of Dienophiles
Characteristics of Dienophiles
Generally, the best dienophiles are alkenes containing electron-withdrawing substituents such as carbonyl, nitrile, and nitro groups. The feasibility of a Diels–Alder reaction depends...


