NH₂OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria

Anammox bacteria produce N₂ gas by oxidizing NH₄⁺ with NO₂^-, and hydroxylamine (NH₂OH) is a potential intermediate of the anammox process. N₂ gas production occurs when anammox bacteria are incubated with NH₂OH only, indicating their capacity for NH₂OH disproportionation with NH₂OH serving as both the electron donor and acceptor. Limited information is currently available on NH₂OH disproportionation by anammox bacteria; therefore, the stoichiometry of anammox bacterial NH₂OH disproportionation was examined in the present study using ¹⁵N-tracing techniques. The anammox bacteria, Brocadia sinica, Jettenia caeni, and Scalindua sp. were incubated with the addition of ¹⁵NH₂OH, and the production of ¹⁵N-labeled nitrogenous compounds was assessed. The anammox bacteria tested performed NH₂OH disproportionation and produced ^15-15N₂ gas and NH₄⁺ as reaction products. The addition of acetylene, an inhibitor of the anammox process, reduced the activity of NH₂OH disproportionation, but not completely. The growth of B. sinica by NH₂OH disproportionation (-240.3‍ ‍kJ mol NH₂OH^-1 under standard conditions) was also tested in 3 up-flow column anammox reactors fed with 1) 0.7‍ ‍mM NH₂OH only, 2) 0.7‍ ‍mM NH₂OH and 0.5‍ ‍mM NH₄⁺, and 3) 0.7‍ ‍mM NH₂OH and 0.5‍ ‍mM NO₂^-. NH₂OH consumption activities were markedly reduced after 7‍ ‍d of operation, indicating that B. sinica was unable to maintain its activity or biomass by NH₂OH disproportionation.