Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

8.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effluent lipopolysaccharide is a prompt marker of peritoneal dialysis-related gram-negative peritonitis.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis·2020
Same author

Current Status and Prospects in the Treatment of Erectile Dysfunction by Adipose-Derived Stem Cells in the Diabetic Animal Model.

Sexual medicine reviews·2020
Same author

Low-Complexity Adaptive Signal Detection for Mobile Molecular Communication.

IEEE transactions on nanobioscience·2020
Same author

Corrigendum to "HMGB1 contributes to adriamycin-induced cardiotoxicity via up-regulating autophagy" [Toxicol. Lett. 292 (2018) 115-121].

Toxicology letters·2019
Same author

Programming nanoparticle valence bonds with single-stranded DNA encoders.

Nature materials·2019
Same author

Novel pH-sensitive films based on starch/polyvinyl alcohol and food anthocyanins as a visual indicator of shrimp deterioration.

International journal of biological macromolecules·2019
Same journal

Shear-Induced CROSS (Cellular RedOx Spreading Shield) Assembly Sustains Neurotrophic Extracellular Vesicle Production for Functional Neural Networks.

Advanced functional materials·2026
Same journal

Buckling-Resistant and Trace-Stacked (BRATS) Design Enables Aid-Free Implantation of Flexible Multielectrode Array with Minimized Inflammatory Tissue Response.

Advanced functional materials·2026
Same journal

Rationally designed anisotropic and auxetic hydrogel patches for adaptation to dynamic organs.

Advanced functional materials·2026
Same journal

Benchtop Fabrication and Integration of Laser-Induced Graphene Strain Gauges and Stimulation Electrodes in Muscle on a Chip Devices.

Advanced functional materials·2026
Same journal

Controlling 3D Contractility via Engineered Fibrous Hydrogel Composites.

Advanced functional materials·2026
Same journal

Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.

Advanced functional materials·2026
See all related articles

Related Experiment Video

Updated: Sep 27, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.6K

Compact Quantum-Dot Microbeads with Sub-Nanometer Emission Linewidth.

Kwon-Hyeon Kim1, Paul H Dannenberg1,2, Hao Yan1

  • 1Wellman Center for Photomedicine and Harvard Medical School, Massachusetts General Hospital, 65 Landsdowne Street, Cambridge, MA 02139, USA.

Advanced Functional Materials
|April 14, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed novel quantum dot microbeads for advanced life science imaging. These stable, bright microbeads exhibit sub-nanometer laser emission, enabling precise multiplexing and in vivo imaging without photobleaching.

Keywords:
bio-imagingcolloidal quantum dotligand exchangemicrobeadmicrolasermultiplexingstimulated emission

More Related Videos

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.3K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.3K

Related Experiment Videos

Last Updated: Sep 27, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.6K
Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.3K
Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

9.3K

Area of Science:

  • Nanotechnology
  • Biophotonics
  • Materials Science

Background:

  • Fluorescent microbeads are crucial tools in life sciences and medical diagnostics.
  • High spectral contrast and sharp photoluminescence are essential for microbead imaging and multiplexing applications.
  • Current microbead technologies face limitations in spectral purity and stability.

Purpose of the Study:

  • To develop novel fluorescent microbeads with enhanced optical properties for advanced imaging.
  • To achieve single-peak laser emission with a sub-nanometer linewidth for improved spectral resolution.
  • To demonstrate the stability and applicability of these microbeads in biological environments.

Main Methods:

  • Fabrication of microbeads using tightly packed and crosslinked quantum dots via ligand exchange.
  • Characterization of optical gain, refractive index, and material stability.
  • Testing of lasing performance, including linewidth and photobleaching, in various environments.
  • Demonstration of intracellular tagging and in vivo multi-color imaging.

Main Results:

  • Demonstrated microbeads capable of single-peak laser emission with a sub-nanometer linewidth.
  • Achieved bright, single-mode lasing with no photobleaching in particles as small as 1.5 μm.
  • Maintained sub-nanometer lasing emission in high-index media like organic solvents and biological tissues.
  • Successfully demonstrated feasibility of intracellular tagging and in vivo multi-color imaging.

Conclusions:

  • Quantum dot microbeads offer superior spectral properties and stability for advanced bioimaging.
  • The developed microbeads enable high-resolution multiplexing and sensitive in vivo diagnostics.
  • These findings pave the way for next-generation fluorescent probes in life sciences and medical applications.