Bioinspired Hydrogen Peroxide-Activated Nanochannels and Their Applications in Cancer Cell Analysis

Bioinspired nanochannels that manipulate ion transport have shown great potential for understanding complex physiological processes. Herein, inspired by the gating function of the biological ion channels, we designed and constructed artificial hydrogen peroxide (H₂O₂)-activated nanochannels by decorating the inner pore surface with 4-(phenoxymethyl) benzeneboronic acid pinacol ester (PBAE). Benefiting from the specific hydrolysis reaction between H₂O₂ and PBAE in the confined nanochannels, the functionalized artificial nanochannels exhibited a highly selective and sensitive response toward H₂O₂. The system could switch between open/closed states in the presence/absence of H₂O₂ by the ionic current test. Meanwhile, comsol simulations were carried out to evidence the mechanism of hydrogen peroxide triggered regulation of ion transport by the nanochannels. It was found that the surface charge density of the nanochannels changed along with the addition of H₂O₂. Furthermore, based on the sensing strategy, the PBAE-functionalized nanochannel membrane was applied in the detection of H₂O₂ in the tumor microenvironment, which achieved highly selective distinguishing of cancerous cells from normal cells. This work provides a versatile method to construct bioinspired nanochannel-based platforms for detecting small reactive molecules and offers prospects for the application of disease diagnosis and prognosis.