Related Experiment Video

Updated: Sep 27, 2025

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

9.9K

Polycomb group ring finger protein 6 suppresses Myc-induced lymphomagenesis

Nina Tanaskovic1, Mattia Dalsass1, Marco Filipuzzi1

  • 1European Institute of Oncology (IEO) - IRCCS, Milan, Italy.

Life Science Alliance
|April 15, 2022
PubMed

Insights

Pcgf6, a subunit of Polycomb repressive complex PRC1.6, unexpectedly suppresses Myc-driven lymphomagenesis independently of Mga and PRC1.6. Its loss accelerates tumor development without altering transcriptional profiles.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Epigenetics

Background:

  • Max protein partners with Myc transcription factors and repressors like Mnt, Mxd1-4, and Mga.
  • Mga is a component of the variant Polycomb repressive complex PRC1.6.
  • PRC1.6 is implicated in antagonizing Myc's role in transcription and oncogenesis.

Purpose of the Study:

  • To investigate the role of Pcgf6, a PRC1.6 subunit, in Myc-induced lymphomagenesis.
  • To determine if Pcgf6's function is dependent on Mga or PRC1.6 in lymphomagenesis.
  • To elucidate the tumor suppressor activity of Pcgf6.

Main Methods:

  • Utilizing Eµ-myc transgenic mice to study lymphomagenesis.
  • Ablation of the Pcgf6 gene in mice.
  • Analysis of transcriptional profiles in pre-tumoral B-cells and lymphomas.

Main Results:

  • Ablation of Pcgf6, but not Mga, accelerated Myc-induced lymphomagenesis.
  • Pcgf6 loss did not significantly impact transcriptional profiles in B-cells or lymphomas.
  • These findings suggest a novel tumor suppressor role for Pcgf6.

Conclusions:

  • Pcgf6 exhibits an unexpected tumor suppressor activity.
  • This tumor suppressor function of Pcgf6 is independent of Mga and PRC1.6.
  • Pcgf6 represents a potential new target for cancer therapies.

More Related Videos

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

8.6K
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

28.2K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.4K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.3K
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.2K