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Single nucleotide detection using bilayer MoS2 nanopores with high efficiency.

Payel Sen1, Manisha Gupta2

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Bilayer molybdenum disulfide (BL MoS2) nanopores offer superior single nucleotide detection compared to monolayer (ML) MoS2. BL MoS2 provides longer dwell times and higher detection rates for DNA sequencing applications.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biophysics

Background:

  • Single nucleotide detection is crucial for disease diagnostics and DNA sequencing.
  • Monolayer (ML) MoS2 nanopores have shown promise for nucleotide identification.
  • Advancements in nanopore technology are needed for improved resolution and efficiency.

Purpose of the Study:

  • To compare the performance of bilayer (BL) MoS2 and ML MoS2 nanopores for single nucleotide detection.
  • To evaluate the efficiency of BL MoS2 nanopores in DNA sequencing.
  • To investigate the impact of nanopore structure on nucleotide translocation dynamics.

Main Methods:

  • Fabrication and characterization of similar-sized ML and BL MoS2 nanopores (<3 nm).
  • Experimental study of single nucleotide and single-stranded DNA translocation events through both nanopore types.
  • Analysis of dwell time, detection rate, and detection efficiency for nucleotide identification.

Main Results:

  • BL MoS2 nanopores exhibited twice the dwell time for single nucleotide detection compared to ML MoS2.
  • The detection rate for BL MoS2 nanopores was five-fold higher (50-60 nucleotides/s) than ML MoS2 (10-15 nucleotides/s).
  • Single nucleotide detection efficiency for BL MoS2 was 89% for DNA, slightly higher than ML MoS2 (85%).

Conclusions:

  • BL MoS2 nanopores demonstrate enhanced performance for single nucleotide detection due to improved analyte/nanopore charge interaction.
  • BL MoS2 nanopores offer higher spatial resolution, longer dwell times, and increased detection rates for DNA sequencing.
  • This study highlights the potential of BL MoS2 nanopores for more efficient and accurate DNA sequencing applications.