Cell cycle arrest of human bronchial epithelial cells modulated by differences in chemical components of particulate...

There is increasing interest in understanding the role of airborne chemical components in modulating the cell cycle of human bronchial epithelial (HBE) cells that is associated with burden of cardiopulmonary disease. To address this need, our study collected ambient PM₁₀ (particles with aerodynamic diameter less than or equal to 10 μm) and PM_2.5 (particles with aerodynamic diameter less than or equal to 2.5 μm) across four sampling sites in Beijing during the year of 2015. Chemical components including organic carbon (OC), elemental carbon (EC), polycyclic aromatic hydrocarbons (PAHs), metals and water soluble ions were determined. Spearman's rank-order correlation was performed to examine the associations between chemical components in ambient particles and cell cycle distributions with p-values adjusted by Bonferroni methodology. Our results demonstrated the significant associations between certain chemical compositions (i.e., PAHs, EC, As and Ni) and percentages of HBE cells in G0/G1 and G1/G2 phases, respectively. Our results highlighted the need to reduce the specific toxins (e.g., PAHs, EC, As and Ni) from ambient particles to protect cardiopulmonary health associated with air pollution. Future study may focus on illustrating the mechanism of certain chemical compositions in altering the cell cycle in HBE cells.