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Updated: Sep 27, 2025

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
Published on: December 23, 2016
A proteolytic nanobiocatalyst with built-in disulphide reducing properties
Manon L Briand1, Maria Bikaki1, Chasper Puorger1
1School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland Hofackerstrasse 30, Muttenz CH-4132 Switzerland patrick.shahgaldian@fhnw.ch.
Abstract:
We report a method to equip proteolytic nanobiocatalysts with intrinsic disulphide bond reducing properties. After immobilisation onto silica particles, selected protease enzymes are partially shielded in a nanometre-thick mercaptosilica layer acting not only as a protective system but also as a substrate reducing agent. The biocatalysts produced efficiently perform simultaneous disulphide bond reduction and protein digestion. Besides a significant simplification of the proteolysis process, this strategy allows for a drastic increase of the enzyme stability.
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