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Related Experiment Video

Updated: Sep 27, 2025

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies
12:55

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

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Ultrasmall copper nanoclusters with multi-enzyme activities.

Yangbin Peng1, Ying Ren2, Hao Zhu1

  • 1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology Wuhan 430070 PR China chang@whut.edu.cn suntl@whut.edu.cn.

RSC Advances
|April 15, 2022
PubMed
Summary

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Glutathione-stabilized copper nanoclusters (CuNCs) mimic key antioxidant enzymes to scavenge reactive oxygen species (ROS). These ultrasmall CuNCs show potential for treating diseases linked to oxidative stress.

Area of Science:

  • Biomaterials
  • Nanotechnology
  • Biochemistry

Background:

  • Reactive oxygen species (ROS) are crucial signaling molecules but excessive levels cause oxidative stress and cell damage.
  • Maintaining cellular homeostasis necessitates effective control of ROS levels.
  • Oxidative stress is implicated in various diseases, highlighting the need for antioxidant therapies.

Purpose of the Study:

  • To develop glutathione (GSH)-stabilized copper nanoclusters (CuNCs) that mimic antioxidant enzyme activity.
  • To investigate the efficacy of CuNCs in scavenging ROS and protecting cells from oxidative stress.

Main Methods:

  • Synthesis of ultrasmall CuNCs (approx. 9 Cu atoms) stabilized by GSH.
  • Enzymatic activity assays to evaluate catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) mimicry.

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  • Intracellular ROS scavenging studies in cells exposed to oxidative stress.

    • Main Results:

    • CuNCs demonstrated efficient H2O2 decomposition (0.23 mg L-1 s-1 at 100 μg mL-1).
    • SOD-like activity reached 25.6 U mg-1 with a 55.4% inhibition rate.
    • CuNCs significantly protected cells from oxidative stress, restoring cell viability to over 90%.

    Conclusions:

    • Ultrasmall GSH-stabilized CuNCs effectively mimic major antioxidant enzymes.
    • CuNCs exhibit potent ROS scavenging capabilities, protecting cells from oxidative damage.
    • These findings suggest promising therapeutic potential for CuNCs in treating ROS-related diseases.