Identification and enhancing production of a novel macrolide compound in engineered Streptomyces peucetius
Van Thuy Thi Pham1, Hue Thi Nguyen1, Chung Thanh Nguyen1
1Department of Life Science and Biochemical Engineering, SunMoon University 70 Sunmoon-ro 221, Tangjeong-myeon Asan-si Chungnam 31460 Republic of Korea jksohng@sunmoon.ac.kr.
Abstract:
Streptomyces peucetius produces doxorubicin and daunorubicin, which are important anticancer drugs. In this study, we activate peucemycin, a new antibacterial compound, using an OSMAC strategy. In general, bioactive compounds are produced in a higher amount at room temperature; however, in this study, we have demonstrated that a bioactive novel compound was successfully activated at a low temperature (18 °C) in S. peucetius DM07. Through LC-MS/MS, IR spectroscopy, and NMR analysis, we identified the structure of this compound as a γ-pyrone macrolide. This compound was found to be novel, thus named peucemycin. It is an unusual 14-membered macrocyclic γ-pyrone ring with cyclization. Also, peucemycin exhibits potential antibacterial activity and a suppressive effect on the viability of various cancer cell lines.
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