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Published on: January 10, 2017
Turn-on signal fluorescence sensor based on DNA derived bio-dots/polydopamine nanoparticles for the detection of
Xiaoxiao Chen1,2, Pu Li1,2, Gaojun Wu1,2
1Xingzhi College, Zhejiang Normal University Lanxi 321100 China hcb@zjnu.cn.
Abstract:
A convenient, fast, sensitive and highly selective fluorescence sensor for the detection of glutathione (GSH) based on DNA derived bio-dots (DNA bio-dots)/polydopamine (PDA) nanoparticles was constructed. The fluorescent switch of DNA bio-dots was induced to turn off because of fluorescence resonance energy transfer (FRET) reactions between DNA bio-dots and PDA. The presence of GSH blocked the spontaneous oxidative polymerization of dopamine (DA) to PDA, leading the fluorescent switch of DNA bio-dots to be "turned on". The degree of fluorescence recovery of DNA bio-dots is linearly correlated with the concentration of GSH within the range of 1.00-100 μmol L-1, and the limit of detection (LOD) is 0.31 μmol L-1 (S/N = 3, n = 9). Furthermore, the fluorescence sensor was successfully used to quantify GSH in human urine and glutathione whitening power, indicating the fluorescence sensor has potential in the detection of human body fluids and pharmaceutical preparations.
