Developing a far-red fluorogenic beta-galactosidase probe for senescent cell imaging and photoablation
Seung Koo Lee1, Zhenhua Shen1, Myung Shin Han1
1Department of Radiology, Molecular Imaging Innovations Institute, Weill Cornell Medicine New York NY 10021 USA cht2018@med.cornell.edu.
Abstract:
A methylene blue (MB)-based beta-galactosidase (β-gal) activatable molecule, Gal-MB, was developed for senescence imaging and light-triggered senolysis. When in contact with LacZ β-gal or senescence-associated β-gal (SA-β-gal), the photoinsensitive Gal-MB becomes fluorescent. Gal-MB also offered selective phototoxicity toward LacZ β-gal expressing cells and drug-induced senescent cells, which express SA-β-gal, after light illumination at 665 nm.
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