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Transport engineering using tobacco transporter NtJAT1 enhances alkaloid production in Escherichia coli
Yasuyuki Yamada1, Akira Nakagawa2, Fumihiko Sato3,4
1Laboratory of Medicinal Cell Biology, Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada-ku, Kobe, Japan.
Bioscience, Biotechnology, and Biochemistry
|April 15, 2022
Abstract:
Transporters have been used in the production of plant metabolites in microorganisms. This study introduced a tobacco multidrug and toxic compound extrusion transporter, NtJAT1, into alkaloid-producing Escherichia coli cells. NtJAT1 expression enhanced alkaloid production secretion into the medium by 14 folds. Our findings further demonstrate the usefulness of the transport-engineering approach.
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