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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Development of aptamer-based ELISA method for d-dimer detection.

Nimet Yildirim-Tirgil1

  • 1Biomedical Engineering Department, Ankara Yildirim Beyazit University, Ankara, Turkey.

Biotechnology and Applied Biochemistry
|April 15, 2022
PubMed
Summary
This summary is machine-generated.

A new aptamer-based ELISA system offers sensitive detection of d-dimer, a marker for severe diseases. This stable and accurate method provides a promising alternative to traditional antibody-based assays for d-dimer analysis.

Keywords:
d -dimer detectionELISAaptamer-based bioanalysisbiotechnology

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Area of Science:

  • Biotechnology
  • Biomedical Diagnostics
  • Molecular Biology

Background:

  • D-dimer is a significant biomarker elevated in various severe diseases.
  • Traditional antibody-based enzyme-linked immunosorbent assays (ELISA) are commonly used for d-dimer detection.
  • Antibodies have limitations including reduced stability and sensitivity in complex sample matrices.

Purpose of the Study:

  • To develop a novel aptamer-based ELISA system for sensitive d-dimer detection.
  • To leverage the advantages of aptamers over antibodies for improved assay performance.
  • To establish a stable and accurate diagnostic tool for d-dimer quantification.

Main Methods:

  • Development of an aptamer-based enzyme-linked immunosorbent assay (ELISA).
  • Utilized aptamers as recognition elements instead of traditional antibodies.
  • Quantification of d-dimer in a broad concentration range (100 ng/ml–10 μg/ml).
  • Assessed assay stability under ambient conditions for extended periods.
  • Validated the system using spiked human serum samples.

Main Results:

  • The aptamer-based ELISA system demonstrated sensitive and quantitative detection of d-dimer.
  • The assay exhibited stability for several months at room temperature.
  • High accuracy was achieved during validation with spiked human serum samples.
  • The developed system operates effectively within a wide dynamic range.

Conclusions:

  • An aptamer-based ELISA system provides a sensitive and stable method for d-dimer detection.
  • Aptamers offer advantages in stability and usability compared to antibodies in ELISA.
  • The developed system shows potential for transformation into a portable and user-friendly d-dimer analysis platform.