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Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo
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Ganglion Cell Layer Thickness Variance Using SPECTRALIS Optical Coherence Tomography.

Paul Mallory1, Sushant Wagley, John Chen

  • 1Department of Ophthalmology and Visual Neuroscience (PM, SW, CMM, PAD, MSL), University of Minnesota, Minneapolis, Minnesota; Departments of Ophthalmology and Neurology (JC), Mayo Clinic, Rochester, Minnesota; and Clinical and Translational Science Institute (BL), University of Minnesota, Minneapolis, Minnesota.

Journal of Neuro-Ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society
|April 15, 2022
PubMed
Summary

The SPECTRALIS optical coherence tomography (OCT) shows reliable mean macular ganglion cell layer (GCL) volume measurements. A variance threshold of 0.07 mm³ or less is predictable, with 0.04 mm³ useful for tracking changes in GCL volume.

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Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Neuroscience

Background:

  • Assessing normal variance in macular ganglion cell layer (GCL) volume is crucial for detecting ocular pathologies.
  • SPECTRALIS optical coherence tomography (OCT) is a key tool for in vivo imaging of the retina.
  • Establishing reliable GCL volume measurement parameters is essential for longitudinal studies.

Purpose of the Study:

  • Determine the normal variance of mean macular ganglion cell layer (GCL) volume using SPECTRALIS OCT.
  • Evaluate the impact of eye registration on GCL volume measurement reproducibility.
  • Establish a reliable threshold for detecting changes in GCL volume.

Main Methods:

  • Utilized SPECTRALIS OCT for high-resolution retinal imaging.
  • Performed repeated GCL volume measurements on 98 eyes without ocular pathology.
  • Employed Bland-Altman analysis, intraclass correlation coefficients (ICCs), and coefficient of repeatability (CR) to assess reproducibility.
  • Compared measurements with and without eye registration (reg-ON vs. reg-OFF).

Main Results:

  • High reproducibility was observed, with ICCs of 0.985 (baseline vs. reg-ON) and 0.977 (baseline vs. reg-OFF).
  • The coefficient of repeatability (CR) was 0.056 mm³ (baseline vs. reg-ON) and 0.069 mm³ (baseline vs. reg-OFF).
  • Ninety percent of eyes showed test-retest reliability within 0.04 mm³.

Conclusions:

  • A predictable threshold of ≤0.07 mm³ for SPECTRALIS OCT mean GCL volume variance was identified in healthy eyes.
  • Eye registration did not significantly alter GCL volume measurement variance.
  • A threshold of 0.04 mm³ is suggested for clinicians to monitor stable versus progressive changes in mean GCL volume.