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Updated: Sep 26, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Classification of Sidr honey and detection of sugar adulteration using right angle fluorescence spectroscopy and
Hina Ali1, Khalid Rafique2, Rahat Ullah1
1Agriculture and Biophotonics Division, National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, Nilore, 45650 Islamabad Pakistan.
Abstract:
Sidr honey is vulnerable to adulteration with low-grade honey and sugar syrups, which compromises its nutritional and medicinal value, demanding fast and reliable analytical tools for quality assessment. In this study, fluorescence spectroscopy was employed to assess the quality of a honey samples, specifically, Sidr, unifloral (Acacia) and multifloral (Acacia, Carisa and Justicia) honey. Fluorescence spectroscopy revealed characteristic spectral signatures of Sidr honey, compared to Acacia and multifloral honey. In addition, cane sugar syrup was artificially added to Sidr honey at different concentrations. These spectral signatures were exploited for the machine-assisted classification of Sidr, sugar syrup and different concentrations of Sidr-sugar mixture. The bagging classification algorithm generated values of sensitivity and specificity close to unity, indicating its ability for efficient discrimination of the samples. Fluorescence spectroscopy in tandem with chemometrics could potentially be used as a rapid analytical tool to identify Sidr honey and its sugar adulteration.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s00217-022-04008-9.
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