Biodegradation of bisphenol A using psychrotolerant bacterial strain Pseudomonas palleroniana GBPI_508

A psychrotolerant bacterial strain of Pseudomonas sp. (P. palleroniana GBPI_508), isolated from the Indian Himalayan region, is studied for analyzing its potential for degrading bisphenol A (BPA). Response surface methodology using Box-Behnken design was used to statistically optimize the environmental factors during BPA degradation and the maximum degradation (97%) was obtained at optimum conditions of mineral salt media pH 9, experimental temperature 25 °C, an inoculum volume of 10% (v/v), and agitation speed 130 rpm at the BPA concentration 270 mg L^-1. The Monod model was used for understanding bacterial degradation kinetics, and 37.5 mg^-1 half saturation coefficient (K_S) and 0.989 regression coefficient (R²) were obtained. Besides, the utmost specific growth rate µmax was witnessed as 0.080 h^-1 with the GBPI_508 during BPA degradation. Metabolic intermediates detected in this study by GC-MS were identified as valeric acid, propionic acid, diglycolic acid, and phenol. The psychrotolerant bacterial strain of Pseudomonas sp. (P. palleroniana GBPI_508), isolated from the Indian Himalayan region has shown good potential for remediation of BPA at variable conditions.