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Detection Efficiency of Ag Nanoparticle Labels for a Heart Failure Marker Using Linear and Square-Wave Anodic
Nikhil Raj1, Richard M Crooks1
1Department of Chemistry, The University of Texas at Austin, 105 E. 24th Street, Stop A5300, Austin, TX 78712-1224, USA.
Biosensors
|April 21, 2022
Summary
Square-wave anodic stripping voltammetry (SWASV) offers superior performance over linear sweep anodic stripping voltammetry (LASV) for nano metalloimmunoassays. SWASV significantly enhances detection efficiency, precision, and lowers the limit of detection for biomarkers like NT-proBNP.
Area of Science:
- Electrochemistry
- Analytical Chemistry
- Biomarker Detection
Background:
- Nano metalloimmunoassays combine antibody interactions with nanoparticle labels for sensitive detection.
- Electrochemical detection on paper devices offers a portable and cost-effective analytical platform.
- Linear sweep anodic stripping voltammetry (LASV) and square-wave anodic stripping voltammetry (SWASV) are electrochemical techniques used for quantitative analysis.
Purpose of the Study:
- To compare the analytical performance of LASV and SWASV for nano metalloimmunoassays.
- To evaluate the impact of SWASV parameter optimization on assay performance.
- To assess the suitability of these techniques for detecting a clinically relevant biomarker, NT-proBNP.
Main Methods:
- Development and testing of two immunoassay formats: a model antibody interaction assay and a sandwich assay for NT-proBNP.
- Utilizing magnetic microbeads and spherical silver nanoparticle labels for antibody conjugation.
- Performing electrochemical detection on a paper-based device using both LASV and SWASV techniques.
- Analyzing figures of merit including measurement precision, calibration sensitivity, and limit of detection (LOD).
Main Results:
- Optimized SWASV demonstrated substantially higher detection efficiency compared to LASV for the NT-proBNP assay.
- SWASV resulted in up to a ~40-fold increase in calibration sensitivity and a ~40% decrease in the coefficient of variation.
- The limit of detection (LOD) for NT-proBNP was reduced to 300.0 pM using SWASV.
- A ~10-fold decrease in LOD was observed for SWASV compared to LASV in a model immunoassay.
Conclusions:
- SWASV is a more effective electrochemical technique than LASV for nano metalloimmunoassays, offering improved sensitivity and precision.
- Optimized SWASV parameters are crucial for maximizing detection efficiency and achieving lower limits of detection.
- The developed SWASV method shows promise for sensitive and quantitative detection of biomarkers like NT-proBNP on paper devices.
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