Pediatric Astrocytomas and Their Association With Polymorphisms in Embryonic Stem Cell Marker Genes
Eduardo Morais de Castro1, Leonardo Vinícius Barbosa1, Aline Simoneti Fonseca1
1Postgraduation Program in Biotechnology Applied in Health of Children and Adolescent of Faculdades Pequeno Príncipe, 245143Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba, Paraná, Brazil.
Background:
Embryonic stem cell markers, such as SOX2, NANOG, and OCT4, are transcription factors expressed in pluripotent stem cells, involved in the mediation of pluripotency and self-renewal. Especially after the discovery of cancer stem cells, these proteins have been associated with several types of neoplasia, including astrocytomas. In the pediatric population, astrocytomas are the most common solid neoplasia and present the highest mortality rates.
Methods:
Our study evaluated 5 polymorphisms in SOX2, NANOG, and POU5F1 genes in 101 pediatric astrocytoma samples.
Results:
We describe the associations between wild and polymorphic alleles in astrocytomas.
Conclusions:
In our results, the intronic polymorphic G allele in SOX2 rs77677339 [G/A] had a borderline association with low-grade astrocytomas, and the intronic polymorphic T allele in NANOG rs10845877 [C/T] showed a higher frequency in grade 2, compared to grade 1 astrocytomas, thus showing promising results.
Impact:
Our study is relevant because it shows a potential correlation between polymorphic embryonic stem cell marker genes and pediatric astrocytomas.
More Related Videos
07:43An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
Published on: August 23, 2016
06:47In Utero Electroporation of Multiaddressable Genome-Integrating Color MAGIC Markers to Individualize Cortical Mouse Astrocytes
Published on: May 21, 2020
