Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

5.9K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.9K
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

12.1K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
12.1K
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.5K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
14.5K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.4K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exosomal circFXR1 drives immune evasion and tumor progression through dual regulation of PD-L1 and mTOR signaling in gastric cancer.

Cancer letters·2026
Same author

SPP1 is required for maintaining mesenchymal cell fate in pancreatic cancer.

Nature·2025
Same author

Depot- and diabetes-specific differences in norepinephrine-mediated adipose tissue angiogenesis, vascular tone, collagen deposition and morphology in obesity.

Life sciences·2022
Same author

Arthur W. Staats (1924-2021).

The American psychologist·2022
Same author

Peritumoral Delivery of Docetaxel-TIPS Microparticles for Prostate Cancer Adjuvant Therapy.

Advanced therapeutics·2021
Same author

Functionalised thermally induced phase separation (TIPS) microparticles enabled for "click" chemistry.

Organic & biomolecular chemistry·2020

Related Experiment Video

Updated: Sep 26, 2025

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.3K

Co-immunoprecipitation Assays.

Ian M Evans1, Ketevan Paliashvili2

  • 1Breast Cancer Research, Cancer Stem Cell Team, Institute of Cancer Research, Chester Beatty Laboratories, London, UK. ian.evans@icr.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2022
PubMed
Summary
This summary is machine-generated.

Co-immunoprecipitation is a common method to identify protein interactions. This guide explains how to perform and analyze this technique, including its limitations.

Keywords:
Protein–protein interactionSignal transductionVEGFp130Cas

More Related Videos

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation
07:16

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Published on: January 5, 2024

1.2K
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

13.0K

Related Experiment Videos

Last Updated: Sep 26, 2025

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

15.3K
Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation
07:16

Author Spotlight: Unraveling the Molecular Mechanisms of Brown and Beige Adipocyte Regulation

Published on: January 5, 2024

1.2K
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

13.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Co-immunoprecipitation (Co-IP) is a widely used biochemical technique.
  • It is employed to identify protein-protein interactions within a biological complex.
  • The method relies on immunoprecipitation to isolate protein complexes.

Purpose of the Study:

  • To provide a comprehensive overview of the co-immunoprecipitation technique.
  • To offer a step-by-step guide for performing co-immunoprecipitation experiments.
  • To discuss the inherent limitations of co-immunoprecipitation.

Main Methods:

  • Utilizing antibodies to precipitate a target protein from a cell lysate.
  • Identifying co-precipitated proteins using techniques like Western blotting.
  • Analyzing the results to confirm protein interactions.

Main Results:

  • Co-immunoprecipitation successfully isolates protein complexes.
  • The technique can determine if two proteins interact in vivo.
  • Limitations include potential for false positives and negatives.

Conclusions:

  • Co-immunoprecipitation is a valuable tool for studying protein interactions.
  • Understanding the technique's principles and limitations is crucial for accurate results.
  • Further analysis is often required to validate protein interactions.