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Simultaneous Measurement of Endothelial Cell Proliferation and Cell Cycle Stage Using Flow Cytometry

Eleanor G Lynam1, James A E Lane1, Elizabeth J T Finding1

  • 1Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2022
PubMed

Keywords:
Cell cycleFlow cytometryProliferationPropidium IodideVEGF

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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