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Development, characterization and in vivo evaluation of the ointment containing hyaluronic acid for potential wound
Thiago Gomes Figueira1, Francisco Vieira Dos Santos2, Sérgio Akinobu Yoshioka1,3
1Inter-units Postgraduate Program in Bioengineering, São Carlos School of Engineering, University of São Paulo, São Carlos, SP, Brazil.
Insights
Hyaluronic acid (HA) extracted from chicken combs effectively promotes wound healing. An ointment containing 1% HA demonstrated improved tissue regeneration and collagen production in vivo.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dermatology
Background:
- Hyaluronic acid (HA) is crucial for wound healing, but its levels decrease with age, impairing tissue regeneration.
- Adults have 50% less HA than newborns, significantly impacting the body's natural healing capabilities.
Purpose of the Study:
- To develop a novel, cost-effective method for extracting HA from chicken combs.
- To formulate and evaluate an ointment containing 1% HA for its efficacy in promoting wound healing in vivo.
Main Methods:
- Extraction of HA from chicken combs using a new, feasible method.
- Formulation of a wound healing ointment with vaseline, lanolin, and 1% HA.
- Rheological analysis of the HA-containing ointment.
- In vivo assessment of wound healing efficacy on days 7 and 14.
Main Results:
- The developed HA extraction method is feasible, low-cost, and easy to implement.
- The HA-containing ointment exhibited viscoelastic properties.
- In vivo tests showed accelerated wound closure by day 7 (0.07 cm² with HA vs. 0.09 cm² without HA).
- Higher collagen content and improved healing were observed in the HA-treated group by day 14.
Conclusions:
- The novel HA extraction method is practical and economical.
- The 1% HA ointment significantly enhances wound healing compared to a non-HA formulation.
- This HA-based ointment shows potential as an effective biomaterial for wound treatment.
Abstract:
Wound healing is a complex biological process. In this context, hyaluronic acid (HA) plays an important role in all phases of wound healing, from inflammation to the remodelling process. Nevertheless, its presence in adults decreases by 50% compared to newborns, which drastically reduces tissue regeneration. In this sense, this work presented a new method of extracting HA from chicken combs, as well as the development and in vivo evaluation of an ointment composed of vaseline, lanolin and HA 1% (w/w) for wound healing. The rheological analysis showed that the ointment containing HA has a viscoelastic behaviour. The in vivo test showed on the 7th day that the group treated with the ointment containing HA had a wound area of 0.07 cm2 against 0.09 cm2 of the ointment without HA (vaseline, and lanolin). On the other hand, the groups treated with the HA ointment had a higher mean percentage of collagen and better healing on the 14th day. The results of this paper indicate that the new method used to obtain HA is feasible, low-cost, and easy to obtain. Furthermore, the HA containing ointment improved wound healing. Therefore, the obtained ointment has great potential for use as an effective biomaterial in wound healing.
