Inducing high nitrite accumulation via modulating nitrate reduction power and carbon flux with Thauera spp. selection

Partial-denitrification (PD, NO₃^--N → NO₂^--N) is emerging as a promising approach for application of anaerobic ammonium oxidation (anammox) process. In this study, stable PD with high nitrite (NO₂^--N) accumulation was achieved by modulating nitrate (NO₃^--N) reduction activity and carbon metabolism. With the influent NO₃^--N increasing from 30 to 200 mg/L, specific NO₃^--N reduction rates (r_no3) were significantly improved, corresponding to the nitrate-to-nitrite transforming ratio (NTR) increasing rapidly to 80.0% within just 70 days. The required COD/NO₃^--N decreased from 4.5 to 2.0 and the carbon flux was more shared in NO₃^--N reduction to NO₂^--N. Notably, Thauera spp. as core denitrifying bacteria was highly enriched with the relative abundance of 70.5%∼82.1% despite different inoculations. This study provided a new insight into inducing high NO₂^--N accumulation and promoting practical application of anammox technology.