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Related Experiment Video

Updated: Sep 26, 2025

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
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Endothelial Progenitor Cell Response to Acute Multicomponent Exercise Sessions with Different Durations.

Suiane Cavalcante1, Manuel Teixeira2, Ana Duarte3

  • 1Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, 4099-002 Porto, Portugal.

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|April 23, 2022
PubMed
Summary

A 30-minute exercise session acutely increases circulating endothelial progenitor cells (EPCs) in adults with cardiovascular risk factors. Longer 45-minute sessions did not show the same benefit, indicating exercise duration impacts vascular health markers.

Keywords:
agingendothelial progenitor cellexerciseflow cytometryvascular health

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Area of Science:

  • Cardiovascular Health
  • Exercise Physiology
  • Endothelial Function

Background:

  • Exercise training is known to benefit vascular health.
  • The specific impact of varying exercise durations on endothelial markers remains unclear.
  • Understanding these effects is crucial for individuals with cardiovascular risk factors.

Purpose of the Study:

  • To investigate the acute effects of different exercise durations (30 min vs. 45 min) on circulating endothelial progenitor cells (EPCs) and endothelial cells (CECs).
  • To assess the impact on vascular health markers in adults with cardiovascular risk factors.

Main Methods:

  • Ten participants with cardiovascular risk factors completed two multicomponent exercise sessions (30 min and 45 min) separated by one week.
  • Blood samples were collected pre- and post-exercise to quantify EPCs and CECs using flow cytometry.

Main Results:

  • A 30-minute exercise session significantly increased circulating EPCs by 1.8% (p = 0.009).
  • A 45-minute session did not yield a significant change in EPCs.
  • Neither exercise duration significantly altered circulating endothelial cells (CECs), suggesting no acute endothelial damage.

Conclusions:

  • A single 30-minute multicomponent exercise session can acutely enhance circulating EPC levels.
  • This duration appears beneficial for vascular health without causing endothelial damage in at-risk adults.
  • Exercise duration is a key factor influencing the acute vascular response to exercise.