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Related Experiment Videos

Biosynthesis and processing of class II histocompatibility antigens.

P Cresswell, J S Blum, D N Kelner

    Critical Reviews in Immunology
    |January 1, 1987
    PubMed
    Summary

    Class II major histocompatibility antigens assemble with the invariant chain early in biosynthesis. These complexes interact with proteoglycans before cell surface expression, with invariant chain dissociation occurring late in the pathway.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Class II major histocompatibility antigens are cell surface heterodimers.
    • Biosynthesis involves association with the invariant (I) chain.

    Purpose of the Study:

    • To elucidate the biosynthetic pathway and assembly of class II major histocompatibility antigens.
    • To identify key intermediates and molecular interactions during antigen processing.

    Main Methods:

    • Analysis of N-linked and O-linked oligosaccharide processing.
    • Investigation of molecular associations using monensin treatment in human B-cell lines.
    • Tracking antigen trafficking through exocytic and endocytic pathways.

    Main Results:

    Related Experiment Videos

  • Invariant chain association occurs early in the endoplasmic reticulum and Golgi apparatus.
  • Class II antigens associate with proteoglycans during transit.
  • Monensin treatment reveals a potential biosynthetic intermediate.
  • Invariant chain dissociation precedes cell surface expression.

    • Conclusions:

    • The invariant chain plays a crucial role in the early assembly and transport of class II antigens.
    • Proteoglycan association is a significant event in the maturation process.
    • The pathway involves an intersection of exocytic and endocytic routes before invariant chain removal.