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Updated: Sep 26, 2025
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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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YY2 Promotes Osteoblast Differentiation by Upregulating Osterix Transcriptional Activity
Meiyu Piao1, Sung Ho Lee1, Myeong Ji Kim1
1College of Pharmacy, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
International Journal of Molecular Sciences
|April 23, 2022
Summary
Yin Yang 2 (YY2) promotes osteoblast differentiation by enhancing Osterix (Osx) gene transcription. This study reveals YY2
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Yin Yang 1 (YY1) is a known transcription factor, but the function of its paralog, Yin Yang 2 (YY2), remains largely undetermined.
- Understanding YY2's role is crucial for deciphering its involvement in cellular processes.
Purpose of the Study:
- To investigate the functional role of YY2 in osteoblast differentiation.
- To elucidate the molecular mechanism by which YY2 influences osteogenesis.
Main Methods:
- Overexpression and knockdown of YY2 in BMP4-induced C2C12 cells.
- Analysis of Osterix (Osx) mRNA and protein levels.
- Evaluation of Osx promoter activity using reporter assays with wild-type and mutated constructs.
Main Results:
- YY2 overexpression enhanced osteoblast differentiation, while YY2 knockdown decreased it.
- YY2 modulated both mRNA and protein levels of Osterix (Osx).
- YY2 significantly increased Osx promoter activity, with regulation occurring at both Y1 and Y2 sites.
Conclusions:
- YY2 acts as a positive regulator of osteoblast differentiation.
- YY2 upregulates osteogenesis by enhancing the transcriptional activity of the Osx gene.
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