Development of Nanocomposite Materials Based on Conductive Polymers for Using in Glucose Biosensor

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Development of reagent-less biosensors for accurate glucose determination is crucial for diabetes management.
• Hybrid nanocomposite conductive polymers offer unique electrochemical properties for biosensor applications.

Purpose of the Study:

• To create a novel amperometric reagent-less biosensor for glucose determination using hybrid nanocomposite conductive polymers.
• To evaluate the performance of nanocomposites based on poly(neutral red) (pNR) and thermally expanded graphite (TEG) for glucose sensing.

Main Methods:

• Electropolymerization of neutral red, thionine, and aniline to form hybrid nanocomposite conductive polymers.
• Characterization of polymer structure using infrared (IR) spectroscopy and scanning electron microscopy (SEM).
• Electrochemical analysis via cyclic voltammetry and impedance spectroscopy.

Main Results:

• The poly(neutral red) (pNR) and thermally expanded graphite (TEG) nanocomposite demonstrated superior electron transfer and interaction with glucose oxidase (GOx).
• The developed glucose biosensor exhibited high sensitivity (1000 ± 200 nA × dm³/mmol) and a low detection limit (0.006 mmol/L).
• The biosensor showed excellent correlation (R² = 0.9828) with standard human blood glucose measurements, with no statistical deviations.

Conclusions:

• The pNR-TEG nanocomposite is a highly promising material for developing sensitive and accurate amperometric reagent-less glucose biosensors.
• The developed biosensor can serve as a viable alternative to conventional glucose testing methods.
• This technology holds potential for creating advanced glucometers and biosensors for other metabolites.