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Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
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A novel polypeptide-modified fluorescent gold nanoclusters for copper ion detection
Hong Zhuang1, Xinyu Jiang1, Sijia Wu1
1College of Food Science and Engineering, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China.
Scientific Reports
|April 23, 2022
Summary
This study developed fluorescent gold nanoclusters (AuNCs) functionalized with a peptide for sensitive copper ion (Cu2+) detection. The novel CCYWDAHRDY-AuNCs probe offers a reliable method for quantifying Cu2+ in biological samples.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Biomolecule-functionalized fluorescent gold nanoclusters (AuNCs) offer biocompatibility and stability.
- Copper ions (Cu2+) play crucial roles in biological systems, and their dysregulation is linked to various diseases.
Purpose of the Study:
- To synthesize and characterize peptide-coupled gold nanoclusters (CCYWDAHRDY-AuNCs).
- To evaluate the fluorescence response of CCYWDAHRDY-AuNCs to Cu2+ for sensitive detection.
Main Methods:
- Synthesis of AuNCs using bovine serum albumin (BSA) as a template in an alkaline aqueous solution.
- Coupling of the peptide CCYWDAHRDY to the synthesized AuNCs.
- Fluorescence spectroscopy to assess the quenching effect of Cu2+ on CCYWDAHRDY-AuNCs.
Main Results:
- The synthesized CCYWDAHRDY-AuNCs demonstrated sensitive fluorescence quenching in the presence of Cu2+.
- Optimal detection conditions were established at pH 6 and 30°C for 10 min.
- A linear detection range for Cu2+ was observed from 0.1 to 4.2 μmol/L with a regression equation y = -105.9x + 693.68 (R² = 0.997).
- The minimum detection limit for Cu2+ was determined to be 52 nmol/L.
Conclusions:
- CCYWDAHRDY-AuNCs serve as a sensitive and reliable fluorescent probe for Cu2+ detection.
- This developed method shows potential for monitoring Cu2+ levels in biological contexts.
