Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

19.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
19.4K
RNA-seq03:21

RNA-seq

10.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.5K
Genomics02:02

Genomics

37.7K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
37.7K
Next-generation Sequencing03:00

Next-generation Sequencing

93.1K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
93.1K
Protein Complex Assembly02:41

Protein Complex Assembly

2.2K
2.2K
Sanger Sequencing01:57

Sanger Sequencing

759.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
759.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

The Biochemical journal·2026
Same author

A new genome assembly of the pea cultivar 'Caméor' provides resources for functional genomics and genetics.

Scientific data·2026
Same author

Complete genetic and epigenetic architecture of D4Z4 macrosatellites in FSHD, BAMS, and reference cohorts with D4Z4End2End.

Genome research·2026
Same author

A comprehensive evaluation of long-read de novo transcriptome assembly.

Genome biology·2026
Same author

<i>Alpseq</i>: an open-source workflow to turbocharge nanobody discovery with high-throughput sequencing.

mAbs·2026
Same author

Pfs48/45 nanobodies block Plasmodium falciparum transmission.

PLoS pathogens·2026

Related Experiment Video

Updated: Sep 26, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K

Assembling Plant Genomes with Long-Read Sequencing.

Quentin Gouil1,2

  • 1Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. gouil.q@wehi.edu.au.

Methods in Molecular Biology (Clifton, N.J.)
|April 24, 2022
PubMed
Summary
This summary is machine-generated.

Advancements in long-read sequencing technology enable the assembly of complex genomes. This study details long-read genome assembly principles using Solanum pennellii nanopore sequencing data.

Keywords:
De novo assemblyGenomeLong-read sequencingNanoporePolishingSolanum pennellii

More Related Videos

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

23.1K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.8K

Related Experiment Videos

Last Updated: Sep 26, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.2K
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

23.1K
Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

8.8K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Long-read sequencing technologies are rapidly advancing, enabling the analysis of large and complex genomes.
  • Previous genome assembly methods faced limitations with repetitive regions and structural variations in complex genomes.

Purpose of the Study:

  • To present the fundamental principles of long-read genome assembly.
  • To illustrate these principles using a practical example of Solanum pennellii genome sequencing.

Main Methods:

  • Utilizing nanopore sequencing for long-read data generation.
  • Applying established and novel bioinformatics algorithms for genome assembly.
  • Comparative analysis of assembly quality and contiguity.

Main Results:

  • Demonstrated the feasibility of assembling a complex plant genome using long-read sequencing.
  • Identified key parameters and potential challenges in long-read genome assembly.
  • Provided a high-quality genome assembly for Solanum pennellii.

Conclusions:

  • Long-read sequencing is a powerful tool for assembling complex genomes.
  • The presented principles offer a framework for future genome assembly projects.
  • This work facilitates further research in Solanum pennellii genomics and related species.