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Updated: Jan 29, 2026

Ookluc: A Plasmodium berghei Line for Identifying Transmission-blocking Compounds
Published on: July 11, 2025
Pfs48/45 nanobodies block Plasmodium falciparum transmission
Frankie M T Lyons1,2, Jill Chmielewski1,2, Mikha Gabriela1,2
1The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia.
None:
Malaria parasite fertilisation occurs within the Anopheles mosquito midgut. Interventions that inhibit parasite fertilisation prevent ongoing transmission and are important for malaria elimination efforts. Pfs48/45 and Pfs230 are two leading transmission-blocking vaccine candidates. Both proteins form a complex on the surface of sexual stage parasites and are essential for male fertility. Here we have identified nanobodies against Pfs48/45 that recognise gametocytes and have strong transmission-reducing activity. The crystal structure of our most potent nanobody in complex with Pfs48/45 reveals it binds a distinct epitope to TB31F, a leading transmission-blocking monoclonal antibody but to similar epitopes as RUPA-44 and RUPA-117. These results demonstrate the potential of nanobodies as a versatile antibody format that can reduce malaria transmission.
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