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Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
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Sphingomyelin localization in the intestinal crypt surface.

Yoshibumi Ueda1, Mitsuhiro Abe2, Toshiyuki Ishiwata3

  • 1Department of Chemistry, School of Science, The University of Tokyo, Tokyo, Japan.

Biochemical and Biophysical Research Communications
|April 26, 2022
PubMed
Summary
This summary is machine-generated.

Researchers visualized sphingomyelin (SM) in living mouse intestines using advanced microscopy. This lipid is found in intestinal crypts and may regulate digestive system functions.

Keywords:
And large intestineEquinatoxin IIFluorescence imagingIn vivo imagingLipidsMouseSmall intestineSphingomyelinTwo-photon microscopy

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Gastroenterology

Background:

  • Macroscopic lipid visualization in live small animals is a significant challenge.
  • Sphingomyelin (SM) plays crucial roles in cellular membranes and signaling pathways.

Purpose of the Study:

  • To develop a method for visualizing sphingomyelin (SM) in the intestines of living mice.
  • To investigate the distribution and potential function of SM in the intestinal crypts.

Main Methods:

  • Utilized two-photon microscopy for in vivo imaging.
  • Employed an SM-binding protein (EqtII-EGFP-His) to detect sphingomyelin.
  • Observed SM distribution in the small and large intestines of live mice.

Main Results:

  • Visualized SM as 10 μm spots within the lamina propria glands of both small and large intestines.
  • Observed vertical penetration of SM spots from the serosa towards the mucosal side.
  • Identified horizontal SM line formations at the mucosal edge of the small intestine and a surface layer in the large intestine, indicating SM covers the crypt membrane.
  • Detected thin SM-positive lines connecting SM spots to blood vessels in the small intestine.

Conclusions:

  • Sphingomyelin is present at both crypt surfaces and within intestinal crypts.
  • The distribution suggests SM plays a role in regulating the functions of the digestive system.