A high selective colorimetric fluorescent probe for detection of silver ions in vitro and in vivo and its application...

To detect silver ions conveniently and rapidly in vitro and in vivo, a selective fluorescent probe with a wide measurement range, AgP, was developed. This probe exhibits a bright green fluorescence with an emission wavelength of 532 nm under 390 nm excitation, and its detection of Ag⁺ is stable in the pH range of 4-10 with a quench-type fluorescence response. Specially, the probe and its easily prepared test strips can be directly used for the colorimetric detection of Ag⁺ in aqueous samples with simple and convenient characteristics, the color change can be observed within a few seconds. The recovery rate of AgP detection water samples was between 117.6% and 98.3%, and the relative standard deviation (RSD) was between 0.41% and 2.06%. AgP is also suitable for in vivo imaging of Ag⁺ in the classical model plant, Arabidopsis thaliana, and 50 μM Ag⁺ can completely quench its fluorescence, which will provide a new detection tool for studying the distribution of Ag⁺ in the environment and live plants.