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Long Noncoding RNA TFAP2A-AS1 Suppressed Hepatitis B Virus Replication by Modulating miR-933/HDAC11

Yu Cheng1, Weiwu Shi2, Xudong Cui1

  • 1Department of Infectious Disease, The Fourth Hospital of Harbin Medical University, Harbin, 150001 Heilongjiang, China.

Disease Markers
|April 28, 2022
PubMed
Abstract

Insights

Long noncoding RNA TFAP2A-AS1 is downregulated in hepatocellular carcinoma (HCC) and suppresses hepatitis B virus (HBV) replication. Upregulation of TFAP2A-AS1 inhibits HBV DNA, HBeAg, and HBsAg expression, suggesting its therapeutic potential.

Area of Science:

  • Molecular Biology
  • Hepatology
  • Oncology

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in various cancers, including hepatocellular carcinoma (HCC).
  • Hepatitis B virus (HBV) infection is a major cause of HCC, and its molecular mechanisms are not fully understood.
  • The role of specific lncRNAs, such as TFAP2A-AS1, in HBV-associated HCC requires further investigation.

Purpose of the Study:

  • To investigate the role of lncRNA TFAP2A-AS1 in hepatocellular carcinoma (HCC) with hepatitis B virus (HBV) infection.
  • To elucidate the regulatory mechanism of TFAP2A-AS1 in HBV replication and HCC progression.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to assess TFAP2A-AS1 and miR-933 levels.
  • Luciferase reporter gene assays to determine interactions between TFAP2A-AS1, miR-933, and HDAC11.
  • Cell proliferation assays (CCK-8) and HBV DNA replication detection (RT-qPCR).

Main Results:

  • TFAP2A-AS1 was downregulated, while miR-933 was upregulated in HCC cell lines and HBV-infected HCC samples.
  • TFAP2A-AS1 functioned as a molecular sponge for miR-933, and HDAC11 was a direct target of miR-933.
  • Overexpression of TFAP2A-AS1 suppressed cell growth, HBV DNA replication, HBeAg, and HBsAg expression, whereas miR-933 promoted these processes.

Conclusions:

  • TFAP2A-AS1 plays a critical role in regulating HBeAg and HBsAg expression and HBV replication.
  • TFAP2A-AS1 may serve as a potential therapeutic target for HBV infection-related HCC.

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