Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

103.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.7K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Longevity and Immunity in Caenorhabditis elegans through Ingestion of Lactiplantibacillus plantarum SKO-001: A Multi-Omics Study.

Food science of animal resources·2026
Same author

Development of a recombinase polymerase amplification-lateral flow assay for rapid visual detection of Meloidogyne hapla.

Archives of microbiology·2026
Same author

Integrative multi-omics analysis reveals systemic and intestinal responses to heat stress in finishing pigs.

Journal of animal science and technology·2026
Same author

A Meta-Analysis of Anti-Thyroid Peroxidase Antibody and Omalizumab Response in Chronic Spontaneous Urticaria.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2026
Same author

Corrigendum to "DNA methylation profiling for a confirmatory test for blood, saliva, semen, vaginal fluid and menstrual blood" [Forensic Sci. Int. Genet. 24 (2016) 75-82].

Forensic science international. Genetics·2026
Same author

Proton Pump Inhibitor Use and Clinical Outcomes in Atrial Fibrillation During Anticoagulation.

Journal of clinical medicine·2026

Related Experiment Video

Updated: Sep 25, 2025

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
07:08

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

6.2K

Transcriptome alterations in spermatogonial stem cells exposed to bisphenol A.

Jin Seop Ahn1, Jong-Hyun Won1, Do-Young Kim1

  • 1Department of Animal Science & Technology, BET Research Institute, Chung-Ang University, Anseong-si, Republic of Korea.

Animal Cells and Systems
|April 28, 2022
PubMed
Summary

Bisphenol A (BPA) exposure negatively impacts male fertility by suppressing spermatogonial stem cell (SSC) proliferation and survival. This study identified key gene expression changes and lysosomal mechanisms affected by BPA, offering insights for fertility preservation.

Keywords:
Bisphenol ARNA sequencingautophagyspermatogonial stem cells

More Related Videos

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

6.1K
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
08:08

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

4.7K

Related Experiment Videos

Last Updated: Sep 25, 2025

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
07:08

Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants

Published on: March 6, 2018

6.2K
Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays
08:28

Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Published on: April 26, 2018

6.1K
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
08:08

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

4.7K

Area of Science:

  • Reproductive Biology
  • Toxicology
  • Genomics

Background:

  • Spermatogonial stem cells (SSCs) are crucial for male fertility and species propagation via spermatogenesis.
  • Increased exposure to plasticizers like bisphenol A (BPA) raises concerns about male reproductive health due to endocrine disruption.
  • BPA is known to impair spermatogenesis and SSC maintenance, but its effects on SSC transcriptomes are not well understood.

Purpose of the Study:

  • To investigate the transcriptome differences in SSCs following exposure to BPA using RNA sequencing.
  • To identify the specific genes and molecular pathways affected by BPA in SSCs.
  • To explore potential mechanisms underlying BPA-induced male infertility.

Main Methods:

  • RNA sequencing (RNA-Seq) was employed to analyze the transcriptomes of SSCs exposed to BPA.
  • Functional annotation and gene set enrichment analysis were performed to interpret the RNA-Seq data.
  • Messenger RNA (mRNA) and protein expression levels were measured to validate the findings.

Main Results:

  • BPA exposure was found to suppress SSC proliferation and survival.
  • Key affected pathways included lysosomal acidification (2 genes) and glycosaminoglycan degradation (5 genes).
  • Differentially expressed genes (DEGs) and their associated molecular mechanisms were identified.

Conclusions:

  • The study identified specific DEGs and lysosomal mechanisms impacted by BPA in SSCs.
  • These findings provide a molecular basis for BPA's detrimental effects on male fertility.
  • The identified DEGs and pathways may offer novel targets for preserving male fertility in the context of environmental exposures.