Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis

Area of Science:

• Biomedical imaging
• Pathology
• Histology

Background:

• 3D tissue clearing is vital for biomedical applications, including human biopsy analysis.
• Immunofluorescence labeling of thick tissues is a slow process.
• Low molecular weight chemical dyes offer potential for faster, high-quality 3D labeling.

Purpose of the Study:

• To evaluate chemical dyes for 3D imaging applications.
• To identify rapid labeling methods for cleared tissues.

Main Methods:

• Tested various chemical dyes for 3D labeling potential.
• Assessed SYTO 16 and eosin combination as a fluorescent H&E alternative.
• Evaluated picrosirius red, Congo red, and fluorescent Nissl stains.

Main Results:

• SYTO 16 and eosin (S&E) showed promise as a fluorescent H&E substitute.
• Picrosirius red, Congo red, and fluorescent Nissl stains demonstrated utility for specific targets.
• Chemical labeling incubation times were under 1 day.

Conclusions:

• Fluorescent chemical dyes can significantly speed up 3D imaging processes.
• These dyes are suitable for various applications, including pathology and neuroscience.
• Chemical dyes offer a viable alternative for rapid 3D fluorescent labeling.