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Circular GOLPH3 RNA exerts oncogenic effects in vitro by regulating the miRNA-1299/LIF axis in oral squamous cell

Chen Zou1, Xia Li1, Haigang Wei1

  • 1School of Medicine, Foshan Stomatological Hospital, Foshan University, Foshan, Guangdong, China.

Bioengineered
|April 28, 2022
PubMed

Insights

Circular GOLPH3 RNA (circGOLPH3) promotes oral squamous cell carcinoma (OSCC) growth, migration, and invasion. Targeting the circGOLPH3/miR-1299/LIF pathway may offer a new therapeutic strategy for OSCC.

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Circular RNAs (circRNAs) are novel noncoding RNAs implicated in biological processes, with aberrant expression potentially driving cancer progression.
  • The specific role of circGOLPH3 in oral squamous cell carcinoma (OSCC) remains largely uncharacterized.
  • Understanding circRNA functions is crucial for developing new cancer therapies.

Purpose of the Study:

  • To investigate the expression and function of circGOLPH3 in oral squamous cell carcinoma (OSCC).
  • To elucidate the underlying molecular mechanism of circGOLPH3 in promoting OSCC progression.
  • To evaluate the therapeutic potential of targeting the circGOLPH3 pathway in OSCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to determine circGOLPH3 expression levels.
  • In vitro gain-of-function and loss-of-function experiments using cell counting kit-8, migration, and invasion assays.
  • In vivo studies and molecular mechanism investigations including western blotting, luciferase activity, and RNA pull-down assays.

Main Results:

  • circGOLPH3 was found to be upregulated in OSCC cells and localized in the cytoplasm with high stability.
  • circGOLPH3 significantly promoted OSCC cell growth, migration, and invasion both in vitro and in vivo.
  • circGOLPH3 acts as a microRNA sponge for miR-1299, leading to the downregulation of LIF expression via the circGOLPH3/miR-1299/LIF axis.

Conclusions:

  • The circGOLPH3/miR-1299/LIF axis plays a critical role in promoting the growth, migration, and invasion of OSCC cells.
  • circGOLPH3 functions as an oncogenic circRNA in OSCC by sponging miR-1299 and upregulating LIF.
  • This axis represents a promising therapeutic target for the treatment of oral squamous cell carcinoma.
Keywords:
CircGOLPH3LIFmiR-1299oncogenic propertiesoral squamous cell carcinoma

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