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Related Experiment Video

Updated: Sep 25, 2025

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

2.0K

Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display.

Lina El Hajji1, Hela Benaissa1, Arnaud Gautier2,3

  • 1Sorbonne Université, École Normale Supérieure, Université PSL, CNRS, Laboratoire des biomolécules, LBM, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2022
PubMed
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This study details a method for developing novel fluorescence-activating proteins using yeast surface display. These engineered proteins act as reporters for gene expression and protein localization in living systems.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Fluorescence-activating proteins are emerging chemogenetic tools for biological research.
  • They enable real-time monitoring of gene expression and protein localization.
  • These proteins fluoresce upon binding specific organic dyes.

Purpose of the Study:

  • To describe a workflow for isolating and engineering fluorescence-activating proteins.
  • To outline a strategy for characterizing the properties of selected protein clones.

Main Methods:

  • Yeast surface display for library construction and screening.
  • Fluorescence-activated cell sorting for iterative selection.
  • Affinity and spectroscopic characterization of engineered proteins.
Keywords:
Fluorescence-activating and absorption-shifting tagsFluorescent chemogenetic reportersFluorogenic chromophores

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Related Experiment Videos

Last Updated: Sep 25, 2025

Protein Engineering by Yeast Surface Display
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Published on: November 29, 2024

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Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
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Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

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Main Results:

  • Successful isolation of functional fluorescence-activating proteins.
  • Demonstration of yeast surface display as an effective engineering platform.
  • Characterization of selected clones' binding and fluorescent properties.

Conclusions:

  • Yeast surface display is a powerful method for engineering fluorescence-activating proteins.
  • This protocol facilitates the development of novel reporters for live-cell imaging.
  • The engineered proteins offer new possibilities for studying biological processes.