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Genome editing and cancer: How far has research moved forward on CRISPR/Cas9?

Saikat Mitra1, Joyatry Sarker2, Anik Mojumder2

  • 1Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|April 28, 2022
PubMed

Insights

Gene editing, particularly CRISPR/Cas9, offers precise tools for understanding cancer metastasis and identifying new drug targets. This technology is revolutionizing research for common cancers like breast, lung, colorectal, and prostate cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Cancer metastasis is a leading cause of mortality, necessitating research into its molecular mechanisms.
  • Identifying novel drug targets is crucial for improving cancer treatment outcomes.
  • Gene editing technologies provide powerful tools for cancer research.

Purpose of the Study:

  • To review gene-editing technologies for cancer research.
  • To compare CRISPR/Cas9, TALEN, and ZFNs, highlighting CRISPR/Cas9's advantages.
  • To explore CRISPR/Cas9 applications in common cancers and future directions.

Main Methods:

  • Review of gene-editing techniques: CRISPR/Cas9, TALEN, and ZFNs.
  • Comparative analysis of gene-editing tool efficiency and precision.
  • Overview of CRISPR/Cas9 applications in breast, lung, colorectal, and prostate cancer research.

Main Results:

  • CRISPR/Cas9 demonstrates superior efficiency and precision compared to TALEN and ZFNs.
  • CRISPR/Cas9 is being actively applied to study molecular mechanisms of common cancers.
  • Recent applications show promise for advancing cancer research and treatment.

Conclusions:

  • CRISPR/Cas9 is a transformative technology for cancer research.
  • Further development and application of CRISPR/Cas9 will accelerate the discovery of cancer drug targets.
  • Addressing current challenges will enhance the clinical utility of gene editing in oncology.
Keywords:
CRISPR/Cas9CancerGenome editingMolecular mechanismsTALEN

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