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Updated: Sep 25, 2025

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Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures
Published on: May 9, 2008
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Establishment of Mouse Epiblast Stem Cells
Pierre Osteil1,2,3
1Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia. pierre.osteil@epfl.ch.
Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2022
Abstract:
Epiblast stem cells are made from the epiblast of mouse post-implantation embryo. They have been critical in the understanding of mammalian pluripotent stem cells as they share similar properties, such as their incapability to contribute to the formation of an embryo after injection into blastocyst. The epiblast stem cells (EpiSC) have delineated a novel status of pluripotency called "primed." How to establish EpiSC from mouse embryo is described in detail in this chapter.

